BVD CONSULT A New Tool for Managing a Challenging Disease
gt;gt; Maintaining and managing the health ofany type of herd animal can present some daunting challenges for producers and veterinarians,when considering diseases such as bovine viral diarrhea, or BVD, in cowcalf operations.Now, those trying to develop strategies to control BVD can rely on a new Internetbasedtool developed at the Kansas State University College of Veterinary Medicine called BVDCONSULT. This BVD management aid was created after Drs. Bob Larson and Brad White decidedthat there was need for the results of BVD research to be more accessible. gt;gt; DR. BOB LARSON: There's been a lot ofwork done on, about BVD itself. It's a virus,
we know a lot about the virus. We know aboutthe diseases that it can cause in cattle and we even know good ways to try to minimizethe impact of that disease in cattle herds. One of the struggles that we have is how doyou implement that in the different types of herd situations that you run into in thereal world. And so we wanted to develop a tool that takes a lot of the works that'sbeen done by scientists all over the world, really, and make that into a decision aidso that producers and their veterinarians can come up with the best specific protocolfor them. gt;gt; Drs. Larson and White worked with facultyfrom the University of Nebraska, Mississippi
State University and Auburn University anddeveloped a basic structure for the program. Envisioning the tool to mimic a conversationbetween a veterinarian and a client, KState veterinary graduate Sherri Merrill washired to help guide the development of the final program. gt;gt; DR. SHERRI MERRILL: It's set up as aseries of questions asking whether or not you have BVD in your herd and then it asksabout different management practices that you can use to either get rid of BVD if youhave it in your herd or keep it out if you don't have it in your herd. And then theproducer has to decide whether or not they
can implement that management practice orcontinue to do so if they've already been doing it. Then they just select, yes, I cando that, or no, I can not. And they'll receive a response based on that question. Then they'llreceive another, move on to the next recommended management practice. gt;gt; DR. BOB LARSON: It is very interactiveand the types of questions are exactly the types of questions that a veterinarian islikely to ask their client. Such as, what is your plan for bringing new animals on thefarmé Are you able to quarantine them and test themé Yes or noé What kind of fencelinecontact do you have with other animalsé A
little, a lot or noneé And then it answersbased on that type of contact what may be the best protocols to protect your herd. gt;gt; While the online tool is meant to be usedwith veterinarian input, it can also be accessed by anyone who has questions related to BVDmanagement. gt;gt; DR. SHERRI MERRILL: On the producer side,I also can see if producers have those questions and maybe they don't have that relationshipwith their veterinarian or they're just wondering about it and are looking for informationon the Internet, then I think they may come across this program and be able to work throughit. Of course, we encourage them to go through
it with their veterinarian and it specificallysays on there, to work closely with your veterinarian and make sure they're involved. gt;gt; Depending on how much interest BVD CONSULTreceives, similar decision tools could be developed for other diseases. gt;gt; DR. BOB LARSON: We think this type of toolcan be really be valuable for a number of different diseases, kind of helping producersand veterinarians ask a series of questions that help them design the best program forthat particular farm. So we would like to take this tool and add other disease to itand maybe trichomoniasis CONSULT or a calf
Bovine viral diarrhoea virus BVDV diagnosis
LDL has developed a technique for the rapid and reliable analysis of ear tissue samples for BVDV diagnosis. The ear tissue samples are checked in the laboratory and sorted into sample racks. The individual samples are then identified by special software and the sample racks are marked with a barcode. Using an opening device, all 96 samples are then opened simultaneously. If a different tissue removal system is used,
the samples are scanned, opened and registered in one single step. The open tubes are processed in a liquid handling machine. The plate barcode ensures the reliable identification of the samples throughout the process. In the first step, 200 microlitres of VIROTYPE TLR lysis buffer is directly pipetted into each tube containing an ear tissue sample. Lysis takes place in two steps. The closed tubes remain inside incubators for 30 minutes at 65 degrees Celsius followed by 15 minutes at 98 degrees Celsius.
After incubation, 10 microlitres of each lysis sample is taken to create pooled samples. The bovine viral diarrhoea virus is detected using the LDL VIROTYPE BVDV test kit. A mix of two components from the kit is automatically transferred to the PCR plate. Afterwards the pooled samples are added. Now realtime RTPCR analysis is carried out. If the results are positive, the individual samples of the positive pools are tested again with the VIROTYPE BVDV test kit. If the findings are negative, retesting isn't necessary. For more information please visit our website.