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From Vaginal Bacteria

Examination of Vaginal Wet Preps

music It's a typical day. A patient has noticedsome itching, or maybe an unpleasant vaginal odor. During her exam, the ian willcheck vaginal pH, examine any discharge that's present, and collect a sample. Then, it'son to the microscope. music This is where you'll gather more specificinformationabout what's causing those vaginal symptoms. We'll show you how to prepare andexamine vaginal wet preps and how to do a whiff test. The results, combined with the patient's vaginal pH test, will aidthe diagnosis.

Under the microscope, you'll be looking for trichomonads, yeast, and the clue cells associated with bacterial vaginosis. We'll show you how to recognize them. music First, the microscope itself: This is a compoundlight microscope. It has several objective lenses on a rotating mount. For our purpose,one of these has to be a 10x low power objective, and one has to be a 40x for greater magnifiion.This flat part, under the objectives, is the stage. Under the stage is the condenser. Belowthat, at the base of the microscope, is the light source. There are two knobs that controlfocus; one for coarse adjustment and one for

fine adjustment. And these are the oculars,or eyepieces. We'll come back to the microscopea minute, but first, let's look at how to prepare wet mount slides. The complete vaginal wet mount involves botha saline prep and a potassium hyoxide, or KOH, prep. When the vaginal sample was collected,the swab was placeda test tube with approximately half a milliliter of saline. So, for the salineprep, you only have to take a op of the suspension and place it on a slide. Add acoverslip, being careful to avoid trapping air bubbles. Your saline slide is ready.Place a second op of the vaginal sample on another slide and add one op of 10 percentKOH. Sniff the preparation immediately, using

your hand to waft any odor toward your nose.This is the whiff test. Note if there's a fishy or amine odor. Then add a coverslip,avoiding air bubbles. Keepmind that you must work quickly to prepare and examine thewet mounts. That's because trichomonads may lose their characteristic motility within15 to 20 minutes. Before we move on now, though, let's lookat the cast of characters you may discover. These are normal squamous epithelial cellsfoundthe vagina. They're large, flat cells with a small nucleus and a large area of cytoplasm.Note that there is some granularitythe cytoplasm.

Polymorphonuclear leukocytes are known asPolys, or PMNs. They may also be called white blood cells, or WBCs. These are small roundcells. Several lobes of the nucleus are visible within the surrounding cell cytoplasm. Findingmany PMNs may indie infection. Trichomonads are pearshaped protozoa whichmove by means of flagella. Trichomonads are similarsize to PMNs and are identifiedby their characteristic jerking movement. The actual flagella may be too thin and toorapidlymoving to be seen. A clue cell is a squamous epithelial cellcoated with enough small bacteria that at least 75 percent of the cell's border is obliterated.It may look as if someone has spread glue

over the cell and pressed itsand. Cluecells are associated with bacterial vaginosis, a conditionwhich the normal microbialflora of the vagina is disrupted. Yeast may be foundtwo forms. Pseudohyphaeare the long, tubular, branching forms. Budding yeast are paired yeast cells that resemblea shoe print. The larger part is the sole and the smaller bud is the heel of the shoe. The saline prep will allow you to see epithelialcells, PMNs, trichomonads, and clue cells. You can also see yeastsaline, but sometimesit's hidden by epithelial cells or by PMNs. Red blood cells, sperm, and bacteria can alsobe seen.

Is A Vaginal Yeast Infection Caused By Bacteria

Hi, Eric Bakker, naturopath, author of CandidaCrusher with another female FAQ. Is a vaginal yeast infection caused by bacteriaƩ No, a yeast infection is caused by yeast andnot bacteria. There's a clear difference between them both. So bacterial infections generallycause a condition called bacterial vaginosis. Candida albicans causes a condition calleda vaginal yeast infection or thrush. So there's a clear distinction between them both. Yeast infections are reasonably normally occurringin many women to a very minor degree where they don't really provide strong symptoms.And quite a high degree of women have Candida

albicans normallythe tissuesthat partof the body. Some have more; some have less, but when it becomes a problem is when it'sovergrowing; it over proliferates. Bacteria are usually keptcheckthatarea, and there are bacteria that livethat part of the body, too. Corynebacterium,for example, are quite commonly foundvaginal tissuethe lining there of the vagina.And they don't usually cause a problem. Lactobacillus species arethere as well, beneficial bacteria.Other bacteria, however, are associated with vaginosis and particularly BV, or bacterialvaginosis. I've spoken about this previously. It causes more of a gray, profuse, watery,a fishy briny sort of discharge; whereas the

vaginal infection is a different infectionaltogether. Both can create an itch, and a vaginal itch is more incessant and can iveyou crazy compared to the bacterial vaginosis. Getting checked out is important here, sogo to your gynecologist or and ask to be swabbed and checked out, so you knowwhat you're dealing with then you can treat it accordingly. You can read a lot more about these conditionsin my book, Candida Crusher, particularly Chapter 5 on chronic vaginal infections. InChapter 3 under diagnosis, I go into great detail more so about the different types ofvaginal infections and inflammations. You

can read a lot more about this. I talk aboutthe medical treatmentsmy book, the natural treatments, and give you lots of solutionson how to deal with this problem. So be sure to get a copy and have a good read there. So vaginal yeast infection is caused by yeastand not bacteria, so I hope that answers your question. Thank you.

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