music It's a typical day. A patient has noticedsome itching, or maybe an unpleasant vaginal odor. During her exam, the ian willcheck vaginal pH, examine any discharge that's present, and collect a sample. Then, it'son to the microscope. music This is where you'll gather more specificinformationabout what's causing those vaginal symptoms. We'll show you how to prepare andexamine vaginal wet preps and how to do a whiff test. The results, combined with the patient's vaginal pH test, will aidthe diagnosis.
Under the microscope, you'll be looking for trichomonads, yeast, and the clue cells associated with bacterial vaginosis. We'll show you how to recognize them. music First, the microscope itself: This is a compoundlight microscope. It has several objective lenses on a rotating mount. For our purpose,one of these has to be a 10x low power objective, and one has to be a 40x for greater magnifiion.This flat part, under the objectives, is the stage. Under the stage is the condenser. Belowthat, at the base of the microscope, is the light source. There are two knobs that controlfocus; one for coarse adjustment and one for
fine adjustment. And these are the oculars,or eyepieces. We'll come back to the microscopea minute, but first, let's look at how to prepare wet mount slides. The complete vaginal wet mount involves botha saline prep and a potassium hyoxide, or KOH, prep. When the vaginal sample was collected,the swab was placeda test tube with approximately half a milliliter of saline. So, for the salineprep, you only have to take a op of the suspension and place it on a slide. Add acoverslip, being careful to avoid trapping air bubbles. Your saline slide is ready.Place a second op of the vaginal sample on another slide and add one op of 10 percentKOH. Sniff the preparation immediately, using
your hand to waft any odor toward your nose.This is the whiff test. Note if there's a fishy or amine odor. Then add a coverslip,avoiding air bubbles. Keepmind that you must work quickly to prepare and examine thewet mounts. That's because trichomonads may lose their characteristic motility within15 to 20 minutes. Before we move on now, though, let's lookat the cast of characters you may discover. These are normal squamous epithelial cellsfoundthe vagina. They're large, flat cells with a small nucleus and a large area of cytoplasm.Note that there is some granularitythe cytoplasm.
Polymorphonuclear leukocytes are known asPolys, or PMNs. They may also be called white blood cells, or WBCs. These are small roundcells. Several lobes of the nucleus are visible within the surrounding cell cytoplasm. Findingmany PMNs may indie infection. Trichomonads are pearshaped protozoa whichmove by means of flagella. Trichomonads are similarsize to PMNs and are identifiedby their characteristic jerking movement. The actual flagella may be too thin and toorapidlymoving to be seen. A clue cell is a squamous epithelial cellcoated with enough small bacteria that at least 75 percent of the cell's border is obliterated.It may look as if someone has spread glue
over the cell and pressed itsand. Cluecells are associated with bacterial vaginosis, a conditionwhich the normal microbialflora of the vagina is disrupted. Yeast may be foundtwo forms. Pseudohyphaeare the long, tubular, branching forms. Budding yeast are paired yeast cells that resemblea shoe print. The larger part is the sole and the smaller bud is the heel of the shoe. The saline prep will allow you to see epithelialcells, PMNs, trichomonads, and clue cells. You can also see yeastsaline, but sometimesit's hidden by epithelial cells or by PMNs. Red blood cells, sperm, and bacteria can alsobe seen.
That Time I Really Hurt My Vagina Waterskiing
Oh man, I just, apparently I hate my vagina, and I just wanna fuckinghit it on everything. (quot;On Tenterhooksquot;) I was about like 14, 15 years old, and this lake we would go to had a comitive slalom skiing course. And I was super intocomitive water skiing
and like wake boarding. So you have to go as quick as you can across the wake of the boat. So the faster the boat is going, the smaller the wake,the faster you can cut. It's all the math that makes it possible. Anyway, we're going fast as hell. I'm cutting back and forth, getting it.
And I hit an edge. I try to save myself, so I over correct, lose my balance. And my back leg comes out of the sleeve. And I just smack into the water as fast and as hard asI ever havemy life, with my legs spread wide open. So many cubic meters of water
just jammed up my vagina and my butt hole, and it hurt so fucking bad. It was literally paralyzing. I could not swim. I was literally justfloating there, crying. I had literally justbeen raped by lake waterboth holes. My mom circles back aroundthe boat,
sees that I'm crying andnot movingthe water. quot;Oh no what's wrong, what's wrongéquot; And I literally just waslike, quot;Just don't touch me. quot;Just don't touch me.quot; And I literally just bobbedthe water for like five minutes as I prepared myself to try and move. Finally, I wasa dull enough pain
where I could move my legs. And I got back up on the boat. And it hurt to sit down, but I sat down. And like literally was just like I, I felt like I wasshock. I was just like, quot;Oh my God.quot; Like I didn't even wannatouch the water anymore. And let's just say, goingto the bathroom that evening
Women Talk About The First Time They Watched Porn
(claps) It's just like (waves) (pop music) The very first timethat I ever saw porn was Third or fourth grade. The guysmy grade had been talking about this thing called whitehouse . I was about eight years old,
and it was a channelthat was offered for free when you ordered Dish NetworkSpanish. And it was PlayboySpanish, and there was terrible dubbing. It was a comition,and I had to sit down and watch quot;Two girls, One Cupquot;. We went into my mom and dad's room, and pulled a tape out, and put it in.
So whitehouse was a quot;pornquot; site that was accessible on the school computer for a short amount of time. And it was my mom anddad having sex. (laughs) If I remember correctly,it was just women and their breasts exposed. I watched this blowjobscene from a far angle, where all you saw was the girl's butt.
It involves two girls and one cup, just like it promises and what they do with this cup I'll just let you find out. What was going throughmy head was confusion. Number one, I was young, I'd never seen people having sex before, ever. And number two, it was my parents, so it was even more confusing.
It felt very taboo. I was like, quot;why is shejust bobbing her headéquot; You can't see a dick,you don't see anything. I think I got through 17 seconds of it. I told her yearslater that we'd seen it, and she was mortified, of course, but she got over it and we had a laugh. When I try to go back to the channel
to learn more about sex, it was blocked. So, my parents blocked it. I don't know if they walkedin on me watching it, sorry.