music It's a typical day. A patient has noticedsome itching, or maybe an unpleasant vaginal odor. During her exam, the ian willcheck vaginal pH, examine any discharge that's present, and collect a sample. Then, it'son to the microscope. music This is where you'll gather more specificinformationabout what's causing those vaginal symptoms. We'll show you how to prepare andexamine vaginal wet preps and how to do a whiff test. The results, combined with the patient's vaginal pH test, will aidthe diagnosis.
Under the microscope, you'll be looking for trichomonads, yeast, and the clue cells associated with bacterial vaginosis. We'll show you how to recognize them. music First, the microscope itself: This is a compoundlight microscope. It has several objective lenses on a rotating mount. For our purpose,one of these has to be a 10x low power objective, and one has to be a 40x for greater magnifiion.This flat part, under the objectives, is the stage. Under the stage is the condenser. Belowthat, at the base of the microscope, is the light source. There are two knobs that controlfocus; one for coarse adjustment and one for
fine adjustment. And these are the oculars,or eyepieces. We'll come back to the microscopea minute, but first, let's look at how to prepare wet mount slides. The complete vaginal wet mount involves botha saline prep and a potassium hyoxide, or KOH, prep. When the vaginal sample was collected,the swab was placeda test tube with approximately half a milliliter of saline. So, for the salineprep, you only have to take a op of the suspension and place it on a slide. Add acoverslip, being careful to avoid trapping air bubbles. Your saline slide is ready.Place a second op of the vaginal sample on another slide and add one op of 10 percentKOH. Sniff the preparation immediately, using
your hand to waft any odor toward your nose.This is the whiff test. Note if there's a fishy or amine odor. Then add a coverslip,avoiding air bubbles. Keepmind that you must work quickly to prepare and examine thewet mounts. That's because trichomonads may lose their characteristic motility within15 to 20 minutes. Before we move on now, though, let's lookat the cast of characters you may discover. These are normal squamous epithelial cellsfoundthe vagina. They're large, flat cells with a small nucleus and a large area of cytoplasm.Note that there is some granularitythe cytoplasm.
Polymorphonuclear leukocytes are known asPolys, or PMNs. They may also be called white blood cells, or WBCs. These are small roundcells. Several lobes of the nucleus are visible within the surrounding cell cytoplasm. Findingmany PMNs may indie infection. Trichomonads are pearshaped protozoa whichmove by means of flagella. Trichomonads are similarsize to PMNs and are identifiedby their characteristic jerking movement. The actual flagella may be too thin and toorapidlymoving to be seen. A clue cell is a squamous epithelial cellcoated with enough small bacteria that at least 75 percent of the cell's border is obliterated.It may look as if someone has spread glue
over the cell and pressed itsand. Cluecells are associated with bacterial vaginosis, a conditionwhich the normal microbialflora of the vagina is disrupted. Yeast may be foundtwo forms. Pseudohyphaeare the long, tubular, branching forms. Budding yeast are paired yeast cells that resemblea shoe print. The larger part is the sole and the smaller bud is the heel of the shoe. The saline prep will allow you to see epithelialcells, PMNs, trichomonads, and clue cells. You can also see yeastsaline, but sometimesit's hidden by epithelial cells or by PMNs. Red blood cells, sperm, and bacteria can alsobe seen.
Episode 6 The Letter Zero
I don't know Mom! Why don't you give me afucking break, oké I had to break up with my boyfriend today. Yeah, because he likesfucking Gossip Girl more than Glee. God! Plus, I have a yeast infection. I don't know, mom,why don't you ask the bread loaf that's bakingmy vaginaé Get me some god damn Monistat! Can I help youé Probably not. What the fuck is that supposed to meané It means whatever the fuck you want it tomean.
Listen, bitch, I don't know who the fuck youthink you are, but I'm Deana. I'm new. I don't give a fuck! Oh reallyé Cuz it sure seems like you do! Well I don't! Well good! Great! Fantastic!
Super! Awesome! Great! Great! Oh my God thank God you're here, this fuckingslut Not right now, Britt. Deana, hi, MackenzieZales, I don't know if you remember me: head cheerleader, homecoming queen, part time model. Yeah. You cursed me outthe bathroom earliertoday.
Oh my god, was that todayé That seems likeso long ago. Right Trishaé Yeah, that was like, literally hours ago. You remember Trisha, righté Not really. Rea reallyé We talked, you pooped, I thoughtwe had a connection. Sorry, uh, nothing. Allow me to introduce you to the coheadvicecaptain of the Overland Park cheer squad, Brittnay Matthews.
Hey. Fuck off. You're the handjob girl, righté What! Fucking Connor Devarnan! Anyway, Deana, I'm glad I ran into you here.We would like to offer you a spot on the Overland Park Cheer Squad. The fuck we would! The megaphone and pompoms are spoken for,but you can either have a baton or the letter
zero. Um, that would be the letter O. Oh. That that makes a lot more sense. She can hold my fucking douche, okay, causeshe's not on this fucking squad. Brittnay! Sidebar! What the fuck is going oné We are at war, Brittnay, and we need allies! Why was I not consulted about thisé
This tutorial provides information about Candida and Candidasis. So let's get started before you begin looking and feelingyounger you must kill off enrich yourself a any candida overgrowththat's growing inside your system it's been said that if you've ever takenantibioticsyour life then you have candida overgrowthyourbody so what is candy to well candida yeast is found naturally inyour digestive system and it worksconjunction with all thefriendly bacteria to keep you healthy but as soon as you can on balancebetween the candied and a friendly
bacteria then the candy to actually ships fromthe east to a fun guess and it starts to invade every a body causing sister symptoms like PMS for fibromyalgia orleaky gut synome or asthma allergies or psoriasis RMSdigestive and urinary problems and advanced agingfatigue it's estimated thatover eighty percent up Canadians and Americans have candidaovergrowth and they don't even know it
if you experience cravings for sweets ona regular basis or feel sick all over or if you takeantibioticsthe past or if you've developed unexplained foodallergies recently than a lot to your health problems maybe associated with candied or use connection the best way to reach your body abolishexcess candy to end to normalize ita healthy way is to follow an alkaline diet